Ascorbic Acid, Pure
Vitamin C, L-ascorbic acid, Ascorbate
CAS Number 50-81-7
EC Number 200-066-2
E number E300
Chemical formula C6H8O6
Solubility in water 330g/l (20 °C)
0.5g of E300 for every 500ml of solution protects against growth of bacteria and lowers solution’s ph protecting against disintegration of compounds.
It’s recommended to use E300 together with E202 which impedes the growth of mildew and fungus.
It is the safest way of conservation for the users and 100% safe for the living organisms and plants in aquariums. Optionally, you can add 2ml of carbon (e.g., easy carbo, liquid carbo) for every 500ml of solution.
10g E300 is enough to conserve 10 liters of solution
Store in dark place, in room temperature.
Good To Know
Ascorbic acid, vitamin C, E300 (acidum ascorbicum)
organic compound belongs to a group of unsaturated polyols. It’s necessary to living organisms for functioning. It’s a vitamin therefore for humans and animals it needs to be delivered with food. E300 is also an antioxidant used as a food additive.
Ascorbic acid naturally occurs as a compound with L configuration in the side chain and with D configuration in the furan formation. Under methanolic NaOH solution ascorbic acid goes through the process of epimerization to erythroboic acid (E315) which can be differentiated due to different side chain configuration. The L-ascorbic acid is sometimes mistakenly called ‘levorotatory vitamin C’ due to its relative configuration confused with optical rotation. In fact, L-ascorbic acid turns polarised light wave to the right. The D-ascorbic acid, on the other hand, is an antioxidant, but it has no biological function – it does not work as a cofactor, therefore it is not a vitamin.
Vitamin C was first isolated in 1928 by Hungarian biochemist Albert Szent-Györgyi from adrenal gland tissue, orange juice and then from peppers. He provided evidence that the newly discovered compound has antiscorbutic properties and called it ‘hexuronic acid’. In 1933, Haworth and Hirst came up with the acid’s structure. Also, Haworth, and independently Reichstein with colleagues, described synthesis of its both enantiomers. In the same year, Szent-Györgyi and Haworth proposed its name to be changed into ‘ascorbic acid’ in order to reflect its antiscorbutic properties. In 1937 both scientists received the Nobel prize for their work on vitamin C: Szent-Györgyi in the field of Physiology or Medicine and Haworth in the field of Chemistry.